A Solvent Tolerant Bacterial Lipase and its Application in Food Industry
We could offer a technology to sysntheize bacterial lipase that has potential applications in the food industry. Brief description of the process / product / technology developed- se to various polar and non-polar organic solvents for 2 h elucidates that the enzyme was stable to all organic solvents tested. The highest relative activity was achieved with chloroform (400%) followed by toluene (250%) and 1-The present invention provides an extra cellular bacterial lipase from Pseudomonas mendocina M-37 (MTCC 7054) with high stability and substrate specificity. The bacteria were isolated from oil industry effluent showing high activity on olive oil. The substrate specificity of Pseudomonas mendocina M-37 lipase shows that the lipase was especially more active towards the synthetic triglycerides and fatty acids esters that possesses butyryl group like benzyl butyrate (1120% relative activity), tributyrin (744%) and amyl butyrate (550%) respectively. The stability of lipase in organic solvents offers advantages for ester synthesis. Exposure of M-37 lipaoctanol (215%).
Sector: Biotechnology
Country: India
Area of Application: The bacterial lipase showing high activity in organic solvents and substrate specificity for butyrated esters has possible significant applications in food industry for ester synthesis. The esterification reactions in food industry are carried out in organic solvents and uses butyrated substrates. Pseudomonas mendocina lipase has possible applications in synthesis of flavour and fragrance esters; for organic synthesis and modification of fats and oils
Keywords: Pseudomonas mendocina, Solvent stable lipase
Advantages: Pseudomonas mendocina lipase possessing high stability in organic solvents, high substrate specificity mainly for butyrated esters has possible significant applications in food industry for ester synthesis.
Environmental aspects: Bio-Degradable and Environmental Friendly
Development Status: Laboratory Model
Legal Protection: Patent
Technical specifications:
Transfer Terms: Consultancy , Technical Services , Technology Licensing
Target Countries: Chile, China, India, New Zealand, Sri Lanka, United States, United Kingdom
Estimated cost (US$):
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Country: India
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