Unique Model for Screening Anti-Diabetic Compounds
The present technology provides a method for generating insulin non-responsive skeletal muscle cell culture model to screen anti-diabetic compounds against insulin resistance. The method comprises: A) Growing myocytes in serum containing medium. B) Differentiating the myocytes into myotubes in a mixture of serum free medium in the continuous presence of insulin. C) Determining the insulin-stimulated tyrosine phosphorylation of Insulin Receptor (IR) and/or Insulin receptor substrate-1 (IRS-1) myotubes differentiated in chronic presence of insulin to that of control (same cells differentiated in a mixture of serum free medium in the absence of insulin), wherein the reduced stimulation of tyrosine phosphorylation of IR and/or IRS-1 in myotubes differentiated in continuous presence of insulin as compared to control, indicated the development of insulin resistance, and D) screening the compounds to determine whether they are capable of enhancing insulin-simulated tyrosine phosphorylation of IR and/or IRS-1 myotubes differentiated in continuous presence of insulin to that of control (same cells are differentiated in the serum free medium in the absence of insulin).
Sector: Pharmaceuticals
Country: India
Area of Application: Pharma industry.
Keywords: diagnostic technology for diabetes; insulin non-responsive skeletal muscle cell culture model to screen anti-diabetic compounds against insulin resistance.
Advantages: First in vitro model of insulin resistance in skeletal muscle. Model is inexpensive. Method is very rapid to screen the compounds as compared to animal models. Results are very reproducible as compared to the animal models. Provide insights into the defects in the signalling mechanisms in insulin resistance. Eliminates the need for the use of animals to screen for the compounds.
Environmental aspects: Not Applicable
Development Status: Laboratory Model
Legal Protection: Patent
Technical specifications:
Transfer Terms: Technology Licensing
Target Countries:
Estimated cost (US$):
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Contact Person: SkyQuest Technology Consulting Pvt. Ltd.
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City: Ahmedabad
Country: India
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